effect of light adjuvant on kinetics of t-cell responses induced by hsv-1 dna immunization
نویسندگان
چکیده
background: studies on efficacy of various vaccines that prevent or reduce the primary and recurrent hsv-1 infection have demonstrated the importance of cellular immunity for protection against the infection. we previously used dna vaccination to induce cellular immunity against hsv-1 infection in mice. objective: the aim of our study was to evaluate the effect of light, a member of tnf super family, on the kinetic of ctl response induced by hsv-1 glycoprotein b based dna vaccine. methods: using a granzyme b elisa for detection and analysis of cd8+ t cells, ctl activity was determined in the spleen of balb/c mice at various time points after primary and booster dose of vaccination. the kinetics of ctl response to primary and secondary hsv-1 infection and dna vaccination were compared to those induced by dna vaccination in combination with light adjuvant in the present study. results: in primary and secondary immunization, the ctl activity in the hsv injected group peaked 7 days and 12 hours post immunization, respectively. after 5 days, light could neither accelerate the ctl response compared to dna vaccination alone nor could enhance the ctl activity in the primary and the first peak of memory response, the amount of granzyme b induced by the light containing vaccine was significantly higher than that induced by the vaccine without the adjuvant. conclusion: although light enhances the cellular response in the booster dose of vaccination, it does not accelerate the ctl response.
منابع مشابه
Effect of LIGHT Adjuvant on Kinetics of T-Cell Responses Induced by HSV-1 DNA Immunization
Background: Studies on efficacy of various vaccines that prevent or reduce the primary and recurrent HSV-1 infection have demonstrated the importance of cellular immunity for protection against the infection. We previously used DNA vaccination to induce cellular immunity against HSV-1 infection in mice. Objective: The aim of our study was to evaluate the effect of LIGHT, a member of TNF super f...
متن کاملinvestigation of effective parameters on the rigidity of light composite diaphragms (psscb) by fem
در این رساله با معرفی سقف های psscb متشکل از ترکیب ورق های فولادی ذوزنقه ای و تخته های سیمانی الیافی به عنوان سقف های پیش ساخته (سازگار با سیستم سازه ای قاب های فولادی سبک) به بررسی پارامترهای موثر بر صلبیت سقف، پرداخته می شود. در تحقیق حاضر ابتدا به مدل سازی دو نمونه سقف آزمایش شده، به روش اجزاء محدود با استفاده از نرم افزار تحلیلی abaqus ver 6.10 پرداخته شده است. نمونه های ساخته شده تحت اعما...
the effect of aqueous extract of garlic on formalin-induced pain in male rats
چکیده اثر عصاره آبی سیر بر درد ناشی از آزمون فرمالین در موش صحرایی نر به کوشش نرگس اسکندری روزبهانی زمینه و هدف: گیاه سیر از خانواده لیلیاسه و گونهallium sativum بومی آسیای میانه بوده و از دوران ،باستان تاکنون به خواص درمانی متفاوت آن مثل: کاهندگی قندخون، کلسترول خون، فشار خون، اثرات مفیدآن بر دستگاه قلبی عروقی و بیماریهای انعقادی خون، اثرات آنتی اکسیداتیو، درمان بیماریهای تنفسی وگوارشی، ا...
15 صفحه اولeffect of oral presentation on development of l2 learners grammar
this experimental study has been conducted to test the effect of oral presentation on the development of l2 learners grammar. but this oral presentation is not merely a deductive instruction of grammatical points, in this presentation two hypotheses of krashen (input and low filter hypotheses), stevicks viewpoints on grammar explanation and correction and widdowsons opinion on limited use of l1...
15 صفحه اولDirect ex vivo kinetic and phenotypic analyses of CD8(+) T-cell responses induced by DNA immunization.
CD8(+) T-cell responses can be induced by DNA immunization, but little is known about the kinetics of these responses in vivo in the absence of restimulation or how soon protective immunity is conferred by a DNA vaccine. It is also unclear if CD8(+) T cells primed by DNA vaccines express the vigorous effector functions characteristic of cells primed by natural infection or by immunization with ...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
iranian journal of immunologyجلد ۸، شماره ۲، صفحات ۷۶-۸۴
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023